NAME Susan Treves
DATE OF BIRTH Sept. 22, 1961
PLACE OF BIRTH Torino, Italy
CITIZENSHIP Italian.
MARITAL STATUS Married to Francesco Zorzato with three children- Sara Zorzato (Toronto, Canada 7/1/1989); Micol Zorzato (Ferrara, Italy 19/12/1990); Daniele Zorzato (Bolzano, Italy 28/8/1995)
CURRENT ADDRESS- Dept of Experimental and Diagnostic Medicine
University of Ferrara
Via Borsari 46
44100 Ferrara. Italy
susan.treves@unife.it
and
Lab 408
Departments of Anaesthesia and Research
Universitätsspital Basel
Hebelstrasse 20,
Basel 4031-CH
Tel. 061-265-2373
e-mail:susan.treves@unibas.ch
EDUCATION
Year place institution degree/certificate
1981 Montreal (Canada) McGill University B.Sc. in "Microbiology & Immunology"
1983 Montreal(Canada) McGill University M.Sc. in "Microbiology & Immunology".
1984 Milano (Italy) University of Milano Laurea in Scienze Biologiche.
1990 Padova (Italy) University of Padova Ph.D.in Molecular & Cellular
Biology & Pathology.
2006 Basel (Switzerland) University of Basel P.D.
PRE and POST-DOCTORAL TRAINING
Prof. D.H. MacLennan, C.H. Best Insitute, University of Toronto, 112 College street, Toronto, Canada (1987-1989).
Dr. T. Steinberg, Dept. Infectious Diseases, Washington University School of Medicine, St. Louis, Missouri, USA (1993).
RESEARCH EXPERIENCE
- Studies on intracellular calcium homeostasis using a variety of intracellular fluorescent calcium indicators.
- Protein purification.
- Isolation and purification of subcellular membranes.
- Biochemical analysis of isolated membrane fractions: enzymatic assays, calcium fluxes, radioligand binding, protein composition, western blotting, ligand overlay.
- Preparation of protein sample for microsequencing and mass spec analysis.
- Production and purification of polyclonal and monoclonal antibodies.
- Molecular biology: RT-PCR, gene cloning, library construction and screening, mutation analysis, plasmid construction, preparation and analysis, Northern and Southern blot. Gene expression in prokarytic and eukaryotic cell systems.
- Analysis of functional properties of expressed proteins by microfluorimetric techniques, radioligand binding assays
- Immunofluorescence and tracking of intracellular organelles by fluorescent and confocal microscopy.
RESEARCH INTERESTS
Molecular and functional characterization of proteins of skeletal muscle sarcoplasmic reticulum in normal and pathological conditions.
Studies of intracellular calcium homeostasis in eukaryotic cells.
RESEARCH GRANTS
Telethon (Italy) 1993, 1994, 1997-
Consiglio Nazionale delle Ricerche (Italy)
SNF N° 3200-967820.02; 3200BO114597
Schweizer Stiftung für due Erforschung der Muskelkrankheiten
Association Francaise contre les Myopathiess (AFM 2004)
TEACHING EXPERIENCE
1982-1983 Teaching assistant, Dept. Microbiology and Immunology, McGill University.
1987 FEBS-CNRS Advanced Course: Dynamics of membrane proteins and cellular energetics (Grenoble and Besançon, France).
1990- 1999 -University of Ferrara School of Medicine, lectures in the courses of General Pathology and Immunology; lectures dealing with the mode of action of bacterial toxins and the Immunology section of the course.
-University of Ferrara Biological sciences, main lecturer and responsible for the course of General Pathology. The course deals with the immune system, with the understanding of the response to tissue injury and general pathological mechanism including, inflammation, atherosclerosis, and wound healing.
1992- date: Tutor for students of the Faculty of Science, University of Padova, Ferrara and Basel, for PhD students in the course of “Cell biology of supramolecular structures” and for students in the PhD program from the Biozentrum, University of Basel.
Predoctoral and Doctoral Students
Mosko Grunberg (Diploma student, Basel University Biozentrum)
Ajuk Anderson (PhD and post-doctoral student)
Thierry Girard (Anaesthesia resident, Department of Anaesthesia, Univeristy Hospital
Basel)
Sylvie Ducreux (PhD student)
Jinyu Xia (Medical Diploma Student)
Mirko Vokcevic (PhD student)
Pubblications by Susan Treves:
Refereed Journals
1) S. Treves and Z. Ali-Khan (1983). Granular and globular acid _-naphthyl-_-acetate esterase staining pattern in resting and mitogen activated murine lymphocytes. Int. Archs Allergy Appl. Immunol. 72: 273-278.
2) S. Treves and Z. Ali-Khan (1984). Kinetics and composition of the inflammatory infiltrates in the solid tumor-like alveolar hydatid cyst. Tropenmed. Parasit. 35: 183-188.
3) S. Treves and Z. Ali-Khan (1984). Characterization of the alveolar hydatid cyst. 2. Cell surface receptors, endocytosed immune complexes and lysosomal enzyme content. Troponmed. Parasit. 35: 231-236.
4) F. Di Virgilio, L.M. Vicentini, S. Treves, G. Riz and T. Pozzan (1985). Inositol phosphate formation in fMet-Leu-Phe stimulated human neutrophils does not require an increase in the cytosolic free Ca2+ concentration. Biochem. J. 229: 361-367.
5) S. Treves, F. Di Virgilio, G. Vaselli and T. Pozzan (1987) Effect of cytochalasins on cytosolic free calcium concentration and phosphoinositide metabolism in leukocytes. Exp. Cell. Research 168: 285-298.
6) S. Treves, F. Di Virgilio, V. Cerundolo, P. Zanovello, D. Collavo and T. Pozzan (1987). Calcium and inositolphosphates in the activation of T-cell mediated cytotoxicity. J. Exp. Med. 166: 33-42.
7) P. Zanovello, A. Rosato, V. Bronte, V. Cerundolo, S. Treves, F. Di Virgilio, T. Pozzan, G. Biasi and D. Collavo (1989). Interaction of lymphokine activated killer cells with susceptible targets does not induce second messenger generation and cytolytic granule exocytosis. J. Exp. Med. 170: 665-677.
8) J.H. Collins, Z. Xi, B.H. Alderson-Lang, S. Treves and P. Volpe (1989). Sequence homology of a canine brain calcium binding protein with calregulin and the human Ross-A antigen. Biochem. Biophys. Res. Comm. 164: 575-579.
9) S. Treves, M. De Mattei, M. Lanfredi, M. Greene, D.H. MacLennan, J. Meldolesi and T. Pozzan (1990). Calreticulin is a candidate for a calsequestrin -like function in Ca++ storage compartiments (calciosomes) of liver and brain. Biochem. J. 271: 473-480.
10) S. Treves, F. Zorzato, P. Chiozzi, P. Melandri, P. Volpe and T. Pozzan (1991). Frog brain expresses a 60 kDa Ca2+ binding protein similar to mammalian calreticulin. Biochem. Biophys. Res. Commun. 175: 444-450.
11) S. Treves, B. Vilsen, P. Chiozzi, J.P. Andersen and F. Zorzato (1992). Molecular cloning, functional expression and tissue distribution of the cDNA encoding frog skeletal muscle calsequestrin. Biochem. J. 283: 762-772.
12) S. Treves, F. Zorzato and T. Pozzan (1992). Identification of calreticulin isoforms in the central nervous system. Biochem. J. 287: 579-581.
13) S. Treves, K.H. Krause, D. Lew and T. Pozzan (1992). Effect of the diacylglycerol kinase inhibitor R59022 on superoxide anion generation, plasma membrane depolarization and Ca2+ fluxes activated by the chemotactic peptide fMLP in human neutrophils. J. Immunol. Res. 4: 181-188.
14) P. Menegazzi, F. Guzzo, B. Baldan, P. Mariani and S. Treves (1993). Purification of calreticulin-like protein(s) from spinach leaves. Biochem. Biophys. Res. Commun. 190: 1130-1135.
15) S. Treves, P. Chiozzi and F. Zorzato (1993) Identification of the domain recognized by anti-(ryanodine receptor) antibodies which affect Ca2+-induced Ca2+ release. Biochem. J. 291: 757-763.
16) F. Zorzato, E. Scutari, V. Tegazzin, E. Clementi and S. Treves (1993). Chlorocresol: an activator of ryanodine receptor-mediated Ca2+ release. Mol. Pharmacol. 44: 1192-1201.
17) S. Treves, P.L. Trentin, M. Ascanelli, G. Bucci and F.Di Virgilio (1994). Apoptosis is dependent on intracellular zinc and independent of intracellular calcium in lymphocytes. Exp. Cell. Res. 211: 339-343.
18) S. Treves, F. Larini., P. Menegazzi, T.H. Steinberg, M. Koval, B. Vilsen, J.P.Andersen and F. Zorzato (1994). Alteration of intracellular Ca2+ in COS-7 cells transfected with the cDNA encoding skeletal muscle ryanodine receptor carrying a mutation associated with malignant hyperthermia. Biochem J. 301: 661-665.
19) P. Menegazzi, F. Larini, S. Treves, R. Guerrini, M. Quadroni and F. Zorzato (1994). Identification and characterization of three calmodulin binding sites of the skeletal muscle ryanodine receptor. Biochemistry 33: 9078-9084.
20) F. Larini, P. Menegazzi, O. Baricordi, F. Zorzato and S. Treves (1995). A ryanodine receptor-like Ca2+ channel is expressed in non-excitable cells. Mol. Pharmacol. 47: 21-28.
21) R. Guerrini, P. Menegazzi, R. Anacardio, M. Marastoni, R. Tomatis, F. Zorzato and S. Treves (1995). Calmodulin binding sites of the skeletal, cardiac and brain ryanodine receptor Ca2+ channels: modulation by the catalytic subunit of cAMP dependent protein kinase? Biochemistry 34: 5120-5129.
22) R. Buratti, G. Prestipino, P. Menegazzi, S. Treves and F. Zorzato (1995). Calcium dependent activation of skeletal muscle Ca2+ release channel (Ryanodine receptor) by calmodulin. Biochem. Biophys. Res. Commun. 213: 1082-1090.
23) V. Tegazzin, E. Scutari, S. Treves and F. Zorzato (1996). Chlorocresol, an additive to commercial succinylcholine induces contracture of human malignant hyperthermia susceptible muscles via activation of the rynodine receptor Ca2+ channel. Anesthesiology 84: 1380-1385.
24) F. Zorzato, P. Menegazzi, S. Treves and M. Ronjat (1996). Role of malignant hyperthermia domain in the regulation of Ca2+ release channel (ryanodine receptor) of skeletal muscle sarcoplasmic reticulum. J. Biol. Chem. 271:22759-22763.
25) S. Treves, E. Scutari, M. Robert, S. Groh, M. Ottolia, G. Prestipino, M. Ronjat and F. Zorzato (1997). Interaction of S100A1 with the Ca2+ release channel (ryanodine receptor) of skeletal muscle. Biochemistry 36: 1496-11503.
26) F. Zorzato, M. Ronjat and S. Treves (1997). Malignant hyperthermia domain in the regulation of Ca2+ release channel (ryanodine receptor). Trends in Cardiovasc. Med. 7: 312- 316.
27) K. Censier, A. Urwyler, F. Zorzato and S. Treves (1998). Intracellular calcium homeostasis in human primary muscle cells from malignant hyperthermia susceptible and normal individuals: effect of overexpression of recombinant wild type and Arg163Cys mutated ryanodine receptors. J. Clin. Invest. 101: 1233-1242.
28) S. Treves, G. Bajocchi, F. Zorzato, M. Govoni and F. Trotta (1998). Identification and characterization of a calreticulin-binding nuclear protein as histone (H1), an autoantigen in systemic lupus erythematosus. Lupus 7: 479-487.
29) B.L. Benacquista, M.R. Sharma, M. Samso’, F. Zorzato, S. Treves and T. Wagenkecht (2000). Amino acid residues 4425-4621 localized on the three dimensional structure of skeletal muscle ryanodine receptor. Biophys. J. 78: 1349-1358.
30) M. Rende, E. Brizi, J. Conner, S. Treves, K. Censier, C. Provenzano, P. Sanna and R. Donato (2000). Cells of myogenic lineage respond to nerve growth factor (NGF) in vitro: a novel role for NGF. Int. J. Devl. Neurosci. 18: 869-885.
31) D. Cavagna, F. Zorzato, E. Babini, G. Prestipino and S. Treves (2000). Methyl p-hydroxybenzoate (E218) a preservative for drugs and food is an activator of the ryanodine receptor Ca2+ release channel. Br. J. Pharmacol. 131: 335.341.
32) F. Zorzato, A.. Anderson, K. Ohlendieck, G. Froemming, R. Guerrini and S. Treves (2000). Identification of a novel 45 kDa protein (JP-45) from rabbit sarcoplasmic reticulum junctional face membrane. Biochem. J. 351:537-543.
33) S. Treves, G. Feriotto, L. Moccagatta, R. Gambari and F. Zorzato (2000). Molecular cloning, expression, fuctional characterization, chromosomal localization and gene structure of junctate a novel integral calcium binding protein of sarco(endo)plasmic reticulum membrane. J. Biol. Chem.275: 39555-39568.
34) T. Girard, A. Urwyler, K. Censier, C. R. Müller, F. Zorzato and S. Treves (2001). Genotype-phenotype comparison of the Swiss malignant hyperthermia population. Hum. Mut. 18: 357-358.
35) N. Tilgen, F. Zorzato, B. Halliger-Keller, F. Muntoni, C. Sewry, L.M. Palmucci, C. Schneider, E. Hauser, F. Lehmann-Horn, C. Müller and S. Treves (2001). Identification of 4 novel mutations in the C-terminal membrane spanning domain of the ryanodine receptor 1: association with central core disease and alteration of calcium homeostasis. Human Mol. Genetics. 25:2879-2887.
36) T. Girard, D. Cavagna, E. Padovan,G. Spagnoli, A. Urwyler, F. Zorzato and S. Treves. (2002) B-lymphocytes from malignant hyperthermia susceptible patients have an increased sensitivity to skeletal muscle ryanodine receptor activators. J. Biol. Chem. 276: 48077-48082.
37) S. Treves, P.Pouliquin, L. Moccagatta and F. Zorzato (2002). Functional properties of EGFP-tagged skeletal muscle calcium release channel (ryanodine receptor) expressed in COS-7 cells: sensitivity to caffeine and 4-chloro-m-cresol. Cell Calcium 31:1-12.
38) T. Girard, S. Treves, K. Censier, C. R. Mueller, F. Zorzato and A. Urwyler (2002). Phenotyping Malignant Hyperthermia Susceptibility by measuring halothane-induced changes in the myoplasmic calcium concentration in cultured human skeletal muscle cells. B.J.A. 89:571-579.
39) G.A. Skeie, A. Mygland, S. Treves, N.E. Gilhus, J.A. Aarli and F. Zorzato (2002). Antibodies against the skeletal muscle sarcoplasmic reticulum Ca2+ release channel (ryanodine receptor) in myasthenia gravis: epitope mapping and functional effect on calcium release from sarcoplasmic reticulum in vitro. Muscle Nerve 27:8 1-89.
40) F. Zorzato, N. Yamaguchi, L. Xu, G. Meissner, C. R. Müller, P. Pouliquin, F. Muntoni, C. Sewry, T. Girard and S. Treves (2003). Clinical and functional effects of a deletion in a COOH-terminal lumenal loop of the skeletal muscle ryanodine receptor. Human Mol. Genetics 12:379-388.
41) A. A. Anderson, S. Treves, D. Biral, R. Betto, D. Sandonà, M. Ronjat and F Zorzato (2003). The novel skeletal muscle sarcoplasmic reticulum JP-45 protein: molecular cloning, tissue distribution, developmental expression and interaction with .1 subunit of the voltage gated calcium channel. J. Biol.Chem. 278:39987-39992.
42) T. Girard, S. Treves, E. Voronkov, M Siegemund and A. Urwyler (2004). Molecular genetic testing for Malignant Hyperthermia susceptibility. Anesthesiology 100:1076-1080
43) S. Treves, C. Franzini-Armstrong, L. Moccagatta, C. Arnoult, C. Grasso, A. Schrum, S. Ducreux, M.X. Zhu, K. Mikoshiba, T. Girard, S. Smida-Rezgui, M. Ronjat and F. Zorzato (2004). Junctate is a key element in calcium entry induced by activation of InsP3 receptors and/or calcium store depletion J. Cell Biol. 166:537-548.
44) S. Ducreux, F. Zorzato, C. R. Müller, C. Sewry, F, Muntoni, R. Quinlivan, G. Restagno, T. Girard and S. Treves (2004). Effect of ryanodine receptor mutations on IL-6 release and intracellular calcium homeostasis in human myotubes from malignant hyperthermia susceptible individuals and patients affected by central core disease. J. Biol.Chem. 279:43838-43846.
45) G. Feriotto, A. Finotti, P. Volpe, S. Treves, S. Ferrari, C. Angelelli, F. Zorzato and R. Gambari (2005). Myocyte Enhancer Factor 2 Activates Promoter Sequences of the Human A{beta}H-J-J Locus, Encoding Aspartyl-{beta}-Hydroxylase, Junctin, and Junctate. Mol. Cell. Biol. 25:3261-3275.
46) A. Divet, S. Paesante, C. Bleuven, A. Anderson, S. Treves and F. Zorzato (2005). Novel sarco(endo)plasmic reticulum proteins and calcium homeostasis in striated muscles. J. Musc Res & Cell Motility 26:7-12.
47) S. Treves, A. Anderson, S. Ducreux, A. Divet, C. Beluven, C. Grasso, S. Paesante and F. Zorzato (2005). Ryanodine receptor 1 mutations, dysregulation of calcium homeostasis and neuromuscular disorders. Neuromuscul. Disord. 15:577-587.
48) S. Stamboulian, M. Moutin, S. Treves, N. Pochon, D. Grunwald, F. Zorzato, M. De Waard, M. Ronjat and C. Arnoult (2005) Junctate an inositol 1,4,5-trisphosphate receptor associated protein, is present in rodent sperm and binds TRPC2 and TRPC5 but not TRPC1 channels. Dev. Biol. 286:326-337.
49) S. Ducreux, F. Zorzato, A. Ferreiro, H. Jungbluth, F. Muntoni, N. Monnier, C. R. Müller, S. Treves (2006) Functional properties of ryanodine receptors carrying 3 amino acid substitutions identified in patients affected by multi-minicore disease and central core disease, expressed in immortalised lymphocytes. Biochem . J. 395:259-266
50) E. Gouadon, R. P. Schuhmeier, D. Ursu, A. Andersen, S. Treves, F. Zorzato, F. Lehmann-Horn and Werner Melzer (2006). A possible role of the junctional face protein JP-45 in modulating Ca2+ release in skeletal muscle. J. Physiol. 572:269-280.
51) A. A. Anderson, X. Altafaj, Z. Zheng, Z. Wang, O. Delbono M. Ronjat, S. Treves and F. Zorzato (2006). The junctional sarcoplasmic reticulum protein JP-45 Affects the functional expression of the voltage dependent calcium channel Cav1.1. J. Cell Sci. 119: 2145-2155.
52) G. Feriotto, A. Finotti, G. Breveglieri, S. Treves, F. Zorzato and R. Gambari (2006). Multiple levels of control of the expression of the human AßH-J-J- locus encoding aspartyl-ß-hydroxylase, junctin and junctate. Ann. N.Y. Acad. Sci. 1091: 184-190.
53) H. Zhou, N. Yamaguchi, L. Xu, Y. Wang, C. Sewry, H. Jungbluth, F. Zorzato, E. Bertini, F. Muntoni, G. Meissner and S. Treves (2006). Characterization of RYR1 mutations in core myopathies. Human Mol. Genetics 15: 2791-2803.
54) A. D. Lyfenko, S. Ducreux, Y. Wang, L. Xu, F. Zorzato, A. Ferreiro, G. Meissner, S. Treves and R. T. Dirksen (2007) Two Central Core Disease (CCD) Deletions in the C-terminal Region of RyR1 Alter Muscle EC Coupling by Distinct Mechanisms. Hum. Mut. 28: 61-68.
55) M. Zhang, T. Yamazaki, M. Yazawa, S. Treves, M. Nishi, M. Murai, E. Shibata, F. Zorzato and H. Takeshima (2007). Calumin, a novel Ca2+-binding transmembrane protein on the endoplasmic reticulum. Cell Calcium . 42:83-90.
56) F. Zorzato, H., Jungbluth, H.,Zhou, F. Muntoni, F. and S. Treves (2007). Functional effects of mutations identified in patients with multiminicore disease. IUBMB Life 59: 14-20.
57) V. Christen, S. Treves, F.H.T. Duong and M. Heim (2007). Activation of endoplasmatic reticulum stress response upregulates protein phosphatase 2A. Hepatology Hepatology 46: 558-565
58) H. Jungbluth, H. Zhou, S. Robb, S. Treves, C. A. Sewry, M. Bitoun, P. Guicheney, A. Buj-Bello, C, Bönnemann and F. Muntoni (2007). Centronuclear myopathy due to a de novo dominant mutation in the skeletal muscle ryanodine receptor (RYR1) gene. Neuromuscul. Disord. 17:338-345.
59) A. Divet, S. Paesante, C. Grasso, D. Cavagna, C. Tiveron, C. Paolini, F. Protasi, C. Huchet-Cadiou, S. Treves and F. Zorzato (2007). Increased Ca2+ storage capacity of the skeletal muscle sarcoplasmic reticulum of transgenic mice over-expressing membrane bound calcium binding protein Junctate. J. Cell Physiol. (in press)
60) H. Zhou, H. Jungbluth, C.A. Sewry, L. Feng, E. Bertini, K. Bushby, V. Straub, H. Roper, M.R. Rose, M. Brockington, M. Kinali, A. Manzur, S. Robb, R. Appleton, S. Messina, A. D’Amico, C.R. Müller, S. Brown, S. Treves, F. Muntoni (2007). Molecular mechanisms and phenotypic variation in RYR1-related congenital myopathies. Brain 130: 2024-2036
61) L. Bracci, M. Vukcevic, G. Spagnoli, S. Ducreux, F. Zorzato, S. Treves (2007) . Ca2+ signaling through ryanodine receptor 1 enhances maturation and activation of human dendritic cells . J. Cell. Sci. 120: 2232-2240.
62) G. Feriotto, A. Finotti, G. Breveglieri, S. Treves, F. Zorzato, R. Gambari (2007). Transcriptional activity and Sp1/3 transcription factor binding to the P1 promotor sequences of the human AßH-J-J locus. FEBS J. 274:4478-4490.
63) O. Delbono, J. Xia, S. Treves, Z.M. Wang, R. Jimenez-Moreno, A.M. Payne, L. Messi, A. Briguet, F. Scharere, M. Nishi, H. Takeshima, F. Zorzato (2007). Loss of skeletal muscle strength by ablation of the sarcoplasmic reticulum protein JP45. Proc. Natl. Acad. Sci. U.S.A. 104: 20108-20113.
64) C. Bleuven, S.Treves, E. Leo, M. Ronjat, M. De Waard, G. Kern, B. Flucher, F. Zorzato (2008). SRP-27 is a novel component of the supramolecular signaling complex involved in skeletal muscle excitation-contraction coupling. Biochem. J. 411:343-349.
65) S.Treves, H. Jungbluth, F. Muntoni, F. Zorzato (2008). Congenital muscle disorders with cores: the ryanodine receptor calcium channel paradigm. Current Opinion Pharmacol. 8: 319-326.
66) M. Vukcevic, C.G. Spagnoli, G. Iezzi, F. Zorzato, S. Treves (2008). Ryanodine receptor activation by Cav1.2 is involved in dendritic cell MHC class II surface expression. J. Biol. Chem. 283:34913-34922.
67) A. Finotti, S. Treves, F. Zorzato, R. Gambari, G. Feriotto (2008). Upstream stimulatory factors are involved in the P1 promoter directed transcription of the AbetaH-J-J locus. BMC Mol Biol. 9:110-119
68) F. Ghassemi, M. Vukcevic, L. Xu, H. Zhou, G. Meissner, F. Muntoni, H. Jungbluth, F. Zorzato, S.Treves (2009). A recessive ryanodine receptor 1 mutation in a CCD patient increases channel activity. Cell Calcium 45:192-197.
69) S. Levano, M. Vukcevic, M. Singer, A. Matter, S. Treves, A. Urwyler, T. Girard (2008). Increasing the Number of Diagnostic Mutations in Malignant Hyperthermia. Hum. Mut. 30:590-598.
70) S Treves, M. Vukceviv, M. Maj, R. Thurnheer, B. Mosca, F. Zorzato (2009). Minor sarcoplasmic reticulum membrane components that modulate excitation-contraction coupling in striated muscles. J. Physiol 587: 3071-3079.
71) M. Vukcevic , M. Broman, G. Islander, M. Bodelsson, E. Ranklev-Twetman , C. R. Müller, S. Treves (2009). Functional Properties of RYR1 Mutations Identified in Swedish Malignant Hyperthermia and Central Core Disease Patients. Anesthesia & Analgesia (in press).
72) H. Zhou, S Lillis, F. Ghassemi, MR Rose, F. Norwood, K. Mills, S. Al-Sarraj, R.J. Lane, L. Feng, CA Sewry, S. Abbs, S Treves, RE. Loy, R. Dirksen, G. Meissner, F .Muntoni, H. Jungbluth (2009). Multi-minicore Disease and atypical periodic paralysis associated with novel mutations in the skeletal muscle ryanodine receptor (RYR1) gene. Neuromuscul. Disord. 20:166-173.
73) M. Vukcevic, F.Zorzato, G. Spagnoli, S. Treves (2010). Frequent calcium oscillations lead to NFAT activation in human immature dendritic cells. J. Biol. Chem . J. Biol. Chem. 285:16003-16011.
74)J.M. Wilmshurst, S. Lillis, H. Zhou, K. Pillay, H. Henderson, W. Kress, C. R. Müller, A. Ndondo, V. Cloke, T. Cullup, E. Bertini, C. Boennemann , V. Straub, R. Quinlivan, J.J. Dowling, S. Al-Sarraj, S. Treves, A.Y. Manzur, C. A. Sewry, F. Muntoni F H. Jungbluth H. (2010). Mutations in RYR1 are a common cause of centronuclear myopathy. Ann Neurol. 68:717-726.
75)S. Treves, J. Griesser, M. Vukcevic, C. Franzini-Armstrong, M.X. Zhu, F. Zorzato. (2010). Agosnist-activated calcium influx occurs at stable plasmalemma-endoplasmic reticulum junctions. J. Cell. Sci. 123:4170-4181.
76)C. Eken, PJ Martin, S Sadallah, S Treves, M Schaller, JA Schifferli. (2010) Ectosomes released by polymorphonuclear Neutrophils induce a MerTK-dependent anti-inflammatory pathway in macrophages. J. Biol. Chem. 285: 39914-39921.
77)S. Treves, M. Vukcevic, PY Jeannet, S. Levano, T. Girard, A. Urwyler, D Fischer, T Voit, H. Jungbluth, S. Lillis, F. Muntoni, R. Quinlivan, A. Sarkozy, K. Bushby, F. Zorzato. (2011) Enhanced excitation coupled Ca2+ entry induces nuclear translocation of NFAT and contributes to IL-6 release from myotubes from patients with Central core disease. Hum. Mol. Genetics 20: 589-600.
78)N.D. Ullrich, D. Fischer, C. Kornblum, M. C. Walter, E. Niggli, F. Zorzato, S. Treves. (2011) Alterations of excitation-contraction coupling and excitation coupled Ca2+ entry in human myotubes carrying CAV3 mutations linked to rippling muscle disease. Hum. Mut. 32:1-9.
79)M. Broman, K. Heinecke, G. Islander, F. Schuster, K. Glahn, M. Bodelsson, S. Treves and C. Müller (2011) Screening of the Ryanodine 1 gene for Malignant Hyperthermia causative mutations by high resolution melt curve analysis. Anesth. Analg. 113:1120-1128.
80) S. Summermatter, R. Thurnheer, G. Santos, B. Mosca, O. Baum, S. Treves, H. Hoppler, F. Zorzato and C. Handschin (2012) Remodeling of calcium handling in skeletal muscle through PGC-1α: Impact on force, fatigability and fiber type. Am. J. Physiol. Cell Physiol. 302: C88-C99.
81) S. Treves, R. Thurnheer, B. Mosca, M. Vukcevic, L. Bergamelli. R. Voltan, V. Oberhauser, L. Csernoch, P. Szentesi and F. Zorzato (2012) SRP-35 a newly identifified protein of the skeletal muscle sarcoplasmic reticulum is a retinol dehydrogenase. Biochem. J. 441: 731-741
82)34. O. Delbono, M.L. Messi, Z.M. Wand, S. Treves, B. Mosca, L. Bergamelli, M. Nishi, H. Takeshima and F. Zorzato (2012). Endogenously determined restriction of food intake overcomes excitation-contraction uncoupling in JP45KO mice with aging. Exp. Gerontol.doi.org/10.1016/j.exger.2012.01.004,
DATE OF BIRTH Sept. 22, 1961
PLACE OF BIRTH Torino, Italy
CITIZENSHIP Italian.
MARITAL STATUS Married to Francesco Zorzato with three children- Sara Zorzato (Toronto, Canada 7/1/1989); Micol Zorzato (Ferrara, Italy 19/12/1990); Daniele Zorzato (Bolzano, Italy 28/8/1995)
CURRENT ADDRESS- Dept of Experimental and Diagnostic Medicine
University of Ferrara
Via Borsari 46
44100 Ferrara. Italy
susan.treves@unife.it
and
Lab 408
Departments of Anaesthesia and Research
Universitätsspital Basel
Hebelstrasse 20,
Basel 4031-CH
Tel. 061-265-2373
e-mail:susan.treves@unibas.ch
EDUCATION
Year place institution degree/certificate
1981 Montreal (Canada) McGill University B.Sc. in "Microbiology & Immunology"
1983 Montreal(Canada) McGill University M.Sc. in "Microbiology & Immunology".
1984 Milano (Italy) University of Milano Laurea in Scienze Biologiche.
1990 Padova (Italy) University of Padova Ph.D.in Molecular & Cellular
Biology & Pathology.
2006 Basel (Switzerland) University of Basel P.D.
PRE and POST-DOCTORAL TRAINING
Prof. D.H. MacLennan, C.H. Best Insitute, University of Toronto, 112 College street, Toronto, Canada (1987-1989).
Dr. T. Steinberg, Dept. Infectious Diseases, Washington University School of Medicine, St. Louis, Missouri, USA (1993).
RESEARCH EXPERIENCE
- Studies on intracellular calcium homeostasis using a variety of intracellular fluorescent calcium indicators.
- Protein purification.
- Isolation and purification of subcellular membranes.
- Biochemical analysis of isolated membrane fractions: enzymatic assays, calcium fluxes, radioligand binding, protein composition, western blotting, ligand overlay.
- Preparation of protein sample for microsequencing and mass spec analysis.
- Production and purification of polyclonal and monoclonal antibodies.
- Molecular biology: RT-PCR, gene cloning, library construction and screening, mutation analysis, plasmid construction, preparation and analysis, Northern and Southern blot. Gene expression in prokarytic and eukaryotic cell systems.
- Analysis of functional properties of expressed proteins by microfluorimetric techniques, radioligand binding assays
- Immunofluorescence and tracking of intracellular organelles by fluorescent and confocal microscopy.
RESEARCH INTERESTS
Molecular and functional characterization of proteins of skeletal muscle sarcoplasmic reticulum in normal and pathological conditions.
Studies of intracellular calcium homeostasis in eukaryotic cells.
RESEARCH GRANTS
Telethon (Italy) 1993, 1994, 1997-
Consiglio Nazionale delle Ricerche (Italy)
SNF N° 3200-967820.02; 3200BO114597
Schweizer Stiftung für due Erforschung der Muskelkrankheiten
Association Francaise contre les Myopathiess (AFM 2004)
TEACHING EXPERIENCE
1982-1983 Teaching assistant, Dept. Microbiology and Immunology, McGill University.
1987 FEBS-CNRS Advanced Course: Dynamics of membrane proteins and cellular energetics (Grenoble and Besançon, France).
1990- 1999 -University of Ferrara School of Medicine, lectures in the courses of General Pathology and Immunology; lectures dealing with the mode of action of bacterial toxins and the Immunology section of the course.
-University of Ferrara Biological sciences, main lecturer and responsible for the course of General Pathology. The course deals with the immune system, with the understanding of the response to tissue injury and general pathological mechanism including, inflammation, atherosclerosis, and wound healing.
1992- date: Tutor for students of the Faculty of Science, University of Padova, Ferrara and Basel, for PhD students in the course of “Cell biology of supramolecular structures” and for students in the PhD program from the Biozentrum, University of Basel.
Predoctoral and Doctoral Students
Mosko Grunberg (Diploma student, Basel University Biozentrum)
Ajuk Anderson (PhD and post-doctoral student)
Thierry Girard (Anaesthesia resident, Department of Anaesthesia, Univeristy Hospital
Basel)
Sylvie Ducreux (PhD student)
Jinyu Xia (Medical Diploma Student)
Mirko Vokcevic (PhD student)
Pubblications by Susan Treves:
Refereed Journals
1) S. Treves and Z. Ali-Khan (1983). Granular and globular acid _-naphthyl-_-acetate esterase staining pattern in resting and mitogen activated murine lymphocytes. Int. Archs Allergy Appl. Immunol. 72: 273-278.
2) S. Treves and Z. Ali-Khan (1984). Kinetics and composition of the inflammatory infiltrates in the solid tumor-like alveolar hydatid cyst. Tropenmed. Parasit. 35: 183-188.
3) S. Treves and Z. Ali-Khan (1984). Characterization of the alveolar hydatid cyst. 2. Cell surface receptors, endocytosed immune complexes and lysosomal enzyme content. Troponmed. Parasit. 35: 231-236.
4) F. Di Virgilio, L.M. Vicentini, S. Treves, G. Riz and T. Pozzan (1985). Inositol phosphate formation in fMet-Leu-Phe stimulated human neutrophils does not require an increase in the cytosolic free Ca2+ concentration. Biochem. J. 229: 361-367.
5) S. Treves, F. Di Virgilio, G. Vaselli and T. Pozzan (1987) Effect of cytochalasins on cytosolic free calcium concentration and phosphoinositide metabolism in leukocytes. Exp. Cell. Research 168: 285-298.
6) S. Treves, F. Di Virgilio, V. Cerundolo, P. Zanovello, D. Collavo and T. Pozzan (1987). Calcium and inositolphosphates in the activation of T-cell mediated cytotoxicity. J. Exp. Med. 166: 33-42.
7) P. Zanovello, A. Rosato, V. Bronte, V. Cerundolo, S. Treves, F. Di Virgilio, T. Pozzan, G. Biasi and D. Collavo (1989). Interaction of lymphokine activated killer cells with susceptible targets does not induce second messenger generation and cytolytic granule exocytosis. J. Exp. Med. 170: 665-677.
8) J.H. Collins, Z. Xi, B.H. Alderson-Lang, S. Treves and P. Volpe (1989). Sequence homology of a canine brain calcium binding protein with calregulin and the human Ross-A antigen. Biochem. Biophys. Res. Comm. 164: 575-579.
9) S. Treves, M. De Mattei, M. Lanfredi, M. Greene, D.H. MacLennan, J. Meldolesi and T. Pozzan (1990). Calreticulin is a candidate for a calsequestrin -like function in Ca++ storage compartiments (calciosomes) of liver and brain. Biochem. J. 271: 473-480.
10) S. Treves, F. Zorzato, P. Chiozzi, P. Melandri, P. Volpe and T. Pozzan (1991). Frog brain expresses a 60 kDa Ca2+ binding protein similar to mammalian calreticulin. Biochem. Biophys. Res. Commun. 175: 444-450.
11) S. Treves, B. Vilsen, P. Chiozzi, J.P. Andersen and F. Zorzato (1992). Molecular cloning, functional expression and tissue distribution of the cDNA encoding frog skeletal muscle calsequestrin. Biochem. J. 283: 762-772.
12) S. Treves, F. Zorzato and T. Pozzan (1992). Identification of calreticulin isoforms in the central nervous system. Biochem. J. 287: 579-581.
13) S. Treves, K.H. Krause, D. Lew and T. Pozzan (1992). Effect of the diacylglycerol kinase inhibitor R59022 on superoxide anion generation, plasma membrane depolarization and Ca2+ fluxes activated by the chemotactic peptide fMLP in human neutrophils. J. Immunol. Res. 4: 181-188.
14) P. Menegazzi, F. Guzzo, B. Baldan, P. Mariani and S. Treves (1993). Purification of calreticulin-like protein(s) from spinach leaves. Biochem. Biophys. Res. Commun. 190: 1130-1135.
15) S. Treves, P. Chiozzi and F. Zorzato (1993) Identification of the domain recognized by anti-(ryanodine receptor) antibodies which affect Ca2+-induced Ca2+ release. Biochem. J. 291: 757-763.
16) F. Zorzato, E. Scutari, V. Tegazzin, E. Clementi and S. Treves (1993). Chlorocresol: an activator of ryanodine receptor-mediated Ca2+ release. Mol. Pharmacol. 44: 1192-1201.
17) S. Treves, P.L. Trentin, M. Ascanelli, G. Bucci and F.Di Virgilio (1994). Apoptosis is dependent on intracellular zinc and independent of intracellular calcium in lymphocytes. Exp. Cell. Res. 211: 339-343.
18) S. Treves, F. Larini., P. Menegazzi, T.H. Steinberg, M. Koval, B. Vilsen, J.P.Andersen and F. Zorzato (1994). Alteration of intracellular Ca2+ in COS-7 cells transfected with the cDNA encoding skeletal muscle ryanodine receptor carrying a mutation associated with malignant hyperthermia. Biochem J. 301: 661-665.
19) P. Menegazzi, F. Larini, S. Treves, R. Guerrini, M. Quadroni and F. Zorzato (1994). Identification and characterization of three calmodulin binding sites of the skeletal muscle ryanodine receptor. Biochemistry 33: 9078-9084.
20) F. Larini, P. Menegazzi, O. Baricordi, F. Zorzato and S. Treves (1995). A ryanodine receptor-like Ca2+ channel is expressed in non-excitable cells. Mol. Pharmacol. 47: 21-28.
21) R. Guerrini, P. Menegazzi, R. Anacardio, M. Marastoni, R. Tomatis, F. Zorzato and S. Treves (1995). Calmodulin binding sites of the skeletal, cardiac and brain ryanodine receptor Ca2+ channels: modulation by the catalytic subunit of cAMP dependent protein kinase? Biochemistry 34: 5120-5129.
22) R. Buratti, G. Prestipino, P. Menegazzi, S. Treves and F. Zorzato (1995). Calcium dependent activation of skeletal muscle Ca2+ release channel (Ryanodine receptor) by calmodulin. Biochem. Biophys. Res. Commun. 213: 1082-1090.
23) V. Tegazzin, E. Scutari, S. Treves and F. Zorzato (1996). Chlorocresol, an additive to commercial succinylcholine induces contracture of human malignant hyperthermia susceptible muscles via activation of the rynodine receptor Ca2+ channel. Anesthesiology 84: 1380-1385.
24) F. Zorzato, P. Menegazzi, S. Treves and M. Ronjat (1996). Role of malignant hyperthermia domain in the regulation of Ca2+ release channel (ryanodine receptor) of skeletal muscle sarcoplasmic reticulum. J. Biol. Chem. 271:22759-22763.
25) S. Treves, E. Scutari, M. Robert, S. Groh, M. Ottolia, G. Prestipino, M. Ronjat and F. Zorzato (1997). Interaction of S100A1 with the Ca2+ release channel (ryanodine receptor) of skeletal muscle. Biochemistry 36: 1496-11503.
26) F. Zorzato, M. Ronjat and S. Treves (1997). Malignant hyperthermia domain in the regulation of Ca2+ release channel (ryanodine receptor). Trends in Cardiovasc. Med. 7: 312- 316.
27) K. Censier, A. Urwyler, F. Zorzato and S. Treves (1998). Intracellular calcium homeostasis in human primary muscle cells from malignant hyperthermia susceptible and normal individuals: effect of overexpression of recombinant wild type and Arg163Cys mutated ryanodine receptors. J. Clin. Invest. 101: 1233-1242.
28) S. Treves, G. Bajocchi, F. Zorzato, M. Govoni and F. Trotta (1998). Identification and characterization of a calreticulin-binding nuclear protein as histone (H1), an autoantigen in systemic lupus erythematosus. Lupus 7: 479-487.
29) B.L. Benacquista, M.R. Sharma, M. Samso’, F. Zorzato, S. Treves and T. Wagenkecht (2000). Amino acid residues 4425-4621 localized on the three dimensional structure of skeletal muscle ryanodine receptor. Biophys. J. 78: 1349-1358.
30) M. Rende, E. Brizi, J. Conner, S. Treves, K. Censier, C. Provenzano, P. Sanna and R. Donato (2000). Cells of myogenic lineage respond to nerve growth factor (NGF) in vitro: a novel role for NGF. Int. J. Devl. Neurosci. 18: 869-885.
31) D. Cavagna, F. Zorzato, E. Babini, G. Prestipino and S. Treves (2000). Methyl p-hydroxybenzoate (E218) a preservative for drugs and food is an activator of the ryanodine receptor Ca2+ release channel. Br. J. Pharmacol. 131: 335.341.
32) F. Zorzato, A.. Anderson, K. Ohlendieck, G. Froemming, R. Guerrini and S. Treves (2000). Identification of a novel 45 kDa protein (JP-45) from rabbit sarcoplasmic reticulum junctional face membrane. Biochem. J. 351:537-543.
33) S. Treves, G. Feriotto, L. Moccagatta, R. Gambari and F. Zorzato (2000). Molecular cloning, expression, fuctional characterization, chromosomal localization and gene structure of junctate a novel integral calcium binding protein of sarco(endo)plasmic reticulum membrane. J. Biol. Chem.275: 39555-39568.
34) T. Girard, A. Urwyler, K. Censier, C. R. Müller, F. Zorzato and S. Treves (2001). Genotype-phenotype comparison of the Swiss malignant hyperthermia population. Hum. Mut. 18: 357-358.
35) N. Tilgen, F. Zorzato, B. Halliger-Keller, F. Muntoni, C. Sewry, L.M. Palmucci, C. Schneider, E. Hauser, F. Lehmann-Horn, C. Müller and S. Treves (2001). Identification of 4 novel mutations in the C-terminal membrane spanning domain of the ryanodine receptor 1: association with central core disease and alteration of calcium homeostasis. Human Mol. Genetics. 25:2879-2887.
36) T. Girard, D. Cavagna, E. Padovan,G. Spagnoli, A. Urwyler, F. Zorzato and S. Treves. (2002) B-lymphocytes from malignant hyperthermia susceptible patients have an increased sensitivity to skeletal muscle ryanodine receptor activators. J. Biol. Chem. 276: 48077-48082.
37) S. Treves, P.Pouliquin, L. Moccagatta and F. Zorzato (2002). Functional properties of EGFP-tagged skeletal muscle calcium release channel (ryanodine receptor) expressed in COS-7 cells: sensitivity to caffeine and 4-chloro-m-cresol. Cell Calcium 31:1-12.
38) T. Girard, S. Treves, K. Censier, C. R. Mueller, F. Zorzato and A. Urwyler (2002). Phenotyping Malignant Hyperthermia Susceptibility by measuring halothane-induced changes in the myoplasmic calcium concentration in cultured human skeletal muscle cells. B.J.A. 89:571-579.
39) G.A. Skeie, A. Mygland, S. Treves, N.E. Gilhus, J.A. Aarli and F. Zorzato (2002). Antibodies against the skeletal muscle sarcoplasmic reticulum Ca2+ release channel (ryanodine receptor) in myasthenia gravis: epitope mapping and functional effect on calcium release from sarcoplasmic reticulum in vitro. Muscle Nerve 27:8 1-89.
40) F. Zorzato, N. Yamaguchi, L. Xu, G. Meissner, C. R. Müller, P. Pouliquin, F. Muntoni, C. Sewry, T. Girard and S. Treves (2003). Clinical and functional effects of a deletion in a COOH-terminal lumenal loop of the skeletal muscle ryanodine receptor. Human Mol. Genetics 12:379-388.
41) A. A. Anderson, S. Treves, D. Biral, R. Betto, D. Sandonà, M. Ronjat and F Zorzato (2003). The novel skeletal muscle sarcoplasmic reticulum JP-45 protein: molecular cloning, tissue distribution, developmental expression and interaction with .1 subunit of the voltage gated calcium channel. J. Biol.Chem. 278:39987-39992.
42) T. Girard, S. Treves, E. Voronkov, M Siegemund and A. Urwyler (2004). Molecular genetic testing for Malignant Hyperthermia susceptibility. Anesthesiology 100:1076-1080
43) S. Treves, C. Franzini-Armstrong, L. Moccagatta, C. Arnoult, C. Grasso, A. Schrum, S. Ducreux, M.X. Zhu, K. Mikoshiba, T. Girard, S. Smida-Rezgui, M. Ronjat and F. Zorzato (2004). Junctate is a key element in calcium entry induced by activation of InsP3 receptors and/or calcium store depletion J. Cell Biol. 166:537-548.
44) S. Ducreux, F. Zorzato, C. R. Müller, C. Sewry, F, Muntoni, R. Quinlivan, G. Restagno, T. Girard and S. Treves (2004). Effect of ryanodine receptor mutations on IL-6 release and intracellular calcium homeostasis in human myotubes from malignant hyperthermia susceptible individuals and patients affected by central core disease. J. Biol.Chem. 279:43838-43846.
45) G. Feriotto, A. Finotti, P. Volpe, S. Treves, S. Ferrari, C. Angelelli, F. Zorzato and R. Gambari (2005). Myocyte Enhancer Factor 2 Activates Promoter Sequences of the Human A{beta}H-J-J Locus, Encoding Aspartyl-{beta}-Hydroxylase, Junctin, and Junctate. Mol. Cell. Biol. 25:3261-3275.
46) A. Divet, S. Paesante, C. Bleuven, A. Anderson, S. Treves and F. Zorzato (2005). Novel sarco(endo)plasmic reticulum proteins and calcium homeostasis in striated muscles. J. Musc Res & Cell Motility 26:7-12.
47) S. Treves, A. Anderson, S. Ducreux, A. Divet, C. Beluven, C. Grasso, S. Paesante and F. Zorzato (2005). Ryanodine receptor 1 mutations, dysregulation of calcium homeostasis and neuromuscular disorders. Neuromuscul. Disord. 15:577-587.
48) S. Stamboulian, M. Moutin, S. Treves, N. Pochon, D. Grunwald, F. Zorzato, M. De Waard, M. Ronjat and C. Arnoult (2005) Junctate an inositol 1,4,5-trisphosphate receptor associated protein, is present in rodent sperm and binds TRPC2 and TRPC5 but not TRPC1 channels. Dev. Biol. 286:326-337.
49) S. Ducreux, F. Zorzato, A. Ferreiro, H. Jungbluth, F. Muntoni, N. Monnier, C. R. Müller, S. Treves (2006) Functional properties of ryanodine receptors carrying 3 amino acid substitutions identified in patients affected by multi-minicore disease and central core disease, expressed in immortalised lymphocytes. Biochem . J. 395:259-266
50) E. Gouadon, R. P. Schuhmeier, D. Ursu, A. Andersen, S. Treves, F. Zorzato, F. Lehmann-Horn and Werner Melzer (2006). A possible role of the junctional face protein JP-45 in modulating Ca2+ release in skeletal muscle. J. Physiol. 572:269-280.
51) A. A. Anderson, X. Altafaj, Z. Zheng, Z. Wang, O. Delbono M. Ronjat, S. Treves and F. Zorzato (2006). The junctional sarcoplasmic reticulum protein JP-45 Affects the functional expression of the voltage dependent calcium channel Cav1.1. J. Cell Sci. 119: 2145-2155.
52) G. Feriotto, A. Finotti, G. Breveglieri, S. Treves, F. Zorzato and R. Gambari (2006). Multiple levels of control of the expression of the human AßH-J-J- locus encoding aspartyl-ß-hydroxylase, junctin and junctate. Ann. N.Y. Acad. Sci. 1091: 184-190.
53) H. Zhou, N. Yamaguchi, L. Xu, Y. Wang, C. Sewry, H. Jungbluth, F. Zorzato, E. Bertini, F. Muntoni, G. Meissner and S. Treves (2006). Characterization of RYR1 mutations in core myopathies. Human Mol. Genetics 15: 2791-2803.
54) A. D. Lyfenko, S. Ducreux, Y. Wang, L. Xu, F. Zorzato, A. Ferreiro, G. Meissner, S. Treves and R. T. Dirksen (2007) Two Central Core Disease (CCD) Deletions in the C-terminal Region of RyR1 Alter Muscle EC Coupling by Distinct Mechanisms. Hum. Mut. 28: 61-68.
55) M. Zhang, T. Yamazaki, M. Yazawa, S. Treves, M. Nishi, M. Murai, E. Shibata, F. Zorzato and H. Takeshima (2007). Calumin, a novel Ca2+-binding transmembrane protein on the endoplasmic reticulum. Cell Calcium . 42:83-90.
56) F. Zorzato, H., Jungbluth, H.,Zhou, F. Muntoni, F. and S. Treves (2007). Functional effects of mutations identified in patients with multiminicore disease. IUBMB Life 59: 14-20.
57) V. Christen, S. Treves, F.H.T. Duong and M. Heim (2007). Activation of endoplasmatic reticulum stress response upregulates protein phosphatase 2A. Hepatology Hepatology 46: 558-565
58) H. Jungbluth, H. Zhou, S. Robb, S. Treves, C. A. Sewry, M. Bitoun, P. Guicheney, A. Buj-Bello, C, Bönnemann and F. Muntoni (2007). Centronuclear myopathy due to a de novo dominant mutation in the skeletal muscle ryanodine receptor (RYR1) gene. Neuromuscul. Disord. 17:338-345.
59) A. Divet, S. Paesante, C. Grasso, D. Cavagna, C. Tiveron, C. Paolini, F. Protasi, C. Huchet-Cadiou, S. Treves and F. Zorzato (2007). Increased Ca2+ storage capacity of the skeletal muscle sarcoplasmic reticulum of transgenic mice over-expressing membrane bound calcium binding protein Junctate. J. Cell Physiol. (in press)
60) H. Zhou, H. Jungbluth, C.A. Sewry, L. Feng, E. Bertini, K. Bushby, V. Straub, H. Roper, M.R. Rose, M. Brockington, M. Kinali, A. Manzur, S. Robb, R. Appleton, S. Messina, A. D’Amico, C.R. Müller, S. Brown, S. Treves, F. Muntoni (2007). Molecular mechanisms and phenotypic variation in RYR1-related congenital myopathies. Brain 130: 2024-2036
61) L. Bracci, M. Vukcevic, G. Spagnoli, S. Ducreux, F. Zorzato, S. Treves (2007) . Ca2+ signaling through ryanodine receptor 1 enhances maturation and activation of human dendritic cells . J. Cell. Sci. 120: 2232-2240.
62) G. Feriotto, A. Finotti, G. Breveglieri, S. Treves, F. Zorzato, R. Gambari (2007). Transcriptional activity and Sp1/3 transcription factor binding to the P1 promotor sequences of the human AßH-J-J locus. FEBS J. 274:4478-4490.
63) O. Delbono, J. Xia, S. Treves, Z.M. Wang, R. Jimenez-Moreno, A.M. Payne, L. Messi, A. Briguet, F. Scharere, M. Nishi, H. Takeshima, F. Zorzato (2007). Loss of skeletal muscle strength by ablation of the sarcoplasmic reticulum protein JP45. Proc. Natl. Acad. Sci. U.S.A. 104: 20108-20113.
64) C. Bleuven, S.Treves, E. Leo, M. Ronjat, M. De Waard, G. Kern, B. Flucher, F. Zorzato (2008). SRP-27 is a novel component of the supramolecular signaling complex involved in skeletal muscle excitation-contraction coupling. Biochem. J. 411:343-349.
65) S.Treves, H. Jungbluth, F. Muntoni, F. Zorzato (2008). Congenital muscle disorders with cores: the ryanodine receptor calcium channel paradigm. Current Opinion Pharmacol. 8: 319-326.
66) M. Vukcevic, C.G. Spagnoli, G. Iezzi, F. Zorzato, S. Treves (2008). Ryanodine receptor activation by Cav1.2 is involved in dendritic cell MHC class II surface expression. J. Biol. Chem. 283:34913-34922.
67) A. Finotti, S. Treves, F. Zorzato, R. Gambari, G. Feriotto (2008). Upstream stimulatory factors are involved in the P1 promoter directed transcription of the AbetaH-J-J locus. BMC Mol Biol. 9:110-119
68) F. Ghassemi, M. Vukcevic, L. Xu, H. Zhou, G. Meissner, F. Muntoni, H. Jungbluth, F. Zorzato, S.Treves (2009). A recessive ryanodine receptor 1 mutation in a CCD patient increases channel activity. Cell Calcium 45:192-197.
69) S. Levano, M. Vukcevic, M. Singer, A. Matter, S. Treves, A. Urwyler, T. Girard (2008). Increasing the Number of Diagnostic Mutations in Malignant Hyperthermia. Hum. Mut. 30:590-598.
70) S Treves, M. Vukceviv, M. Maj, R. Thurnheer, B. Mosca, F. Zorzato (2009). Minor sarcoplasmic reticulum membrane components that modulate excitation-contraction coupling in striated muscles. J. Physiol 587: 3071-3079.
71) M. Vukcevic , M. Broman, G. Islander, M. Bodelsson, E. Ranklev-Twetman , C. R. Müller, S. Treves (2009). Functional Properties of RYR1 Mutations Identified in Swedish Malignant Hyperthermia and Central Core Disease Patients. Anesthesia & Analgesia (in press).
72) H. Zhou, S Lillis, F. Ghassemi, MR Rose, F. Norwood, K. Mills, S. Al-Sarraj, R.J. Lane, L. Feng, CA Sewry, S. Abbs, S Treves, RE. Loy, R. Dirksen, G. Meissner, F .Muntoni, H. Jungbluth (2009). Multi-minicore Disease and atypical periodic paralysis associated with novel mutations in the skeletal muscle ryanodine receptor (RYR1) gene. Neuromuscul. Disord. 20:166-173.
73) M. Vukcevic, F.Zorzato, G. Spagnoli, S. Treves (2010). Frequent calcium oscillations lead to NFAT activation in human immature dendritic cells. J. Biol. Chem . J. Biol. Chem. 285:16003-16011.
74)J.M. Wilmshurst, S. Lillis, H. Zhou, K. Pillay, H. Henderson, W. Kress, C. R. Müller, A. Ndondo, V. Cloke, T. Cullup, E. Bertini, C. Boennemann , V. Straub, R. Quinlivan, J.J. Dowling, S. Al-Sarraj, S. Treves, A.Y. Manzur, C. A. Sewry, F. Muntoni F H. Jungbluth H. (2010). Mutations in RYR1 are a common cause of centronuclear myopathy. Ann Neurol. 68:717-726.
75)S. Treves, J. Griesser, M. Vukcevic, C. Franzini-Armstrong, M.X. Zhu, F. Zorzato. (2010). Agosnist-activated calcium influx occurs at stable plasmalemma-endoplasmic reticulum junctions. J. Cell. Sci. 123:4170-4181.
76)C. Eken, PJ Martin, S Sadallah, S Treves, M Schaller, JA Schifferli. (2010) Ectosomes released by polymorphonuclear Neutrophils induce a MerTK-dependent anti-inflammatory pathway in macrophages. J. Biol. Chem. 285: 39914-39921.
77)S. Treves, M. Vukcevic, PY Jeannet, S. Levano, T. Girard, A. Urwyler, D Fischer, T Voit, H. Jungbluth, S. Lillis, F. Muntoni, R. Quinlivan, A. Sarkozy, K. Bushby, F. Zorzato. (2011) Enhanced excitation coupled Ca2+ entry induces nuclear translocation of NFAT and contributes to IL-6 release from myotubes from patients with Central core disease. Hum. Mol. Genetics 20: 589-600.
78)N.D. Ullrich, D. Fischer, C. Kornblum, M. C. Walter, E. Niggli, F. Zorzato, S. Treves. (2011) Alterations of excitation-contraction coupling and excitation coupled Ca2+ entry in human myotubes carrying CAV3 mutations linked to rippling muscle disease. Hum. Mut. 32:1-9.
79)M. Broman, K. Heinecke, G. Islander, F. Schuster, K. Glahn, M. Bodelsson, S. Treves and C. Müller (2011) Screening of the Ryanodine 1 gene for Malignant Hyperthermia causative mutations by high resolution melt curve analysis. Anesth. Analg. 113:1120-1128.
80) S. Summermatter, R. Thurnheer, G. Santos, B. Mosca, O. Baum, S. Treves, H. Hoppler, F. Zorzato and C. Handschin (2012) Remodeling of calcium handling in skeletal muscle through PGC-1α: Impact on force, fatigability and fiber type. Am. J. Physiol. Cell Physiol. 302: C88-C99.
81) S. Treves, R. Thurnheer, B. Mosca, M. Vukcevic, L. Bergamelli. R. Voltan, V. Oberhauser, L. Csernoch, P. Szentesi and F. Zorzato (2012) SRP-35 a newly identifified protein of the skeletal muscle sarcoplasmic reticulum is a retinol dehydrogenase. Biochem. J. 441: 731-741
82)34. O. Delbono, M.L. Messi, Z.M. Wand, S. Treves, B. Mosca, L. Bergamelli, M. Nishi, H. Takeshima and F. Zorzato (2012). Endogenously determined restriction of food intake overcomes excitation-contraction uncoupling in JP45KO mice with aging. Exp. Gerontol.doi.org/10.1016/j.exger.2012.01.004,
